ASI SponsorRossi, GiannaGiannaRossiGasperi, ValeriaValeriaGasperiParo, RitaRitaParo2020-09-172020-09-172007-03-01https://hdl.handle.net/20.500.13025/2222Among the biological activities of the endocannabinoid anandamide (N-arachidonoylethanolamine) (AEA), growing interest has been attracted by the regulation of mammalian fertility. Recently we have shown that treatment of mouse primary Sertoli cells with FSH enhances the activity of the AEA hydrolase [fatty acid amide hydrolase (FAAH)], though the molecular details were not elucidated. Here, we investigated whether FSH was also able to affect the enzymes that synthesize AEA (N-acyltransferase and N-acyl-phosphatidyl-ethanolamine-phospholipase D), the endogenous content of this endocannabinoid, and the level of the AEA-binding vanilloid receptor 1 (transient receptor potential channel vanilloid receptor subunit 1). We show that FSH enhanced FAAH activity (up to approximately 500% of the controls) and expression (up to approximately 300%), leading to a marked reduction (down to approximately 15%) of AEA content. However N-acyltransferase and N-acyl-phosphatidyl-ethanolamine-phospholipase D activity, and transient receptor potential channel vanilloid receptor subunit 1 binding were not affected. We also show that diacylglycerol lipase and monoacylglycerol lipase, which respectively synthesize and degrade 2-arachidonoyl-glycerol, were not regulated by FSH, neither was the membrane transport of this endocannabinoid. In addition, we show that FAAH stimulation by FSH was abrogated by inhibitors of protein kinase A (PKA) and cytochrome-P(450) aromatase, and was conversely mimicked by N,O-dibutyryl cAMP and estrogen. Finally, we demonstrate that FSH protects Sertoli cells against the pro-apoptotic activity of AEA, through PKA and aromatase-dependent activation of FAAH. Altogether these data suggest that FAAH is the only target of FSH among the elements of the endocannabinoid system, and that its regulation by PKA and aromatase-dependent pathways impacts Sertoli cell proliferation.AmidohydrolasesAmidohydrolases: metabolismAnimalsApoptosisApoptosis: drug effectsArachidonic AcidsArachidonic Acids: biosynthesisArachidonic Acids: metabolismAromataseAromatase: physiologyBiologicalCellsCulturedCyclic AMP-Dependent Protein KinasesCyclic AMP-Dependent Protein Kinases: physiologyFollicle Stimulating HormoneFollicle Stimulating Hormone: pharmacologyGlyceridesGlycerides: metabolismInbred StrainsMaleMiceModelsPolyunsaturated AlkamidesProtein BindingSertoli CellsSertoli Cells: drug effectsSertoli Cells: metabolismSignal TransductionSignal Transduction: drug effectsTRPV Cation ChannelsTRPV Cation Channels: metabolismjournal article54dcce098580fe1368eeb218http://endo.endojournals.org/cgi/content/abstract/148/3/143154dcce098580fe1368eeb218