High levels of anandamide, an endogenous cannabinoid, block the growth of sheep preimplantation embryos by inducing apoptosis and reversible arrest of cell proliferation.

ASI SponsorTurco, M YM YTurcoMatsukawa, KKMatsukawaCzernik, MMCzernik2020-09-172020-09-172008-10-01https://hdl.handle.net/20.500.13025/2732The process of implantation is mediated by various molecules, one of which is anandamide (AEA), a lipid signalling ligand belonging to the family of endocannabinoids. AEA exerts its effects on implantation by binding to the Type 1 Cannabinoid Receptor (CB1-R), expressed in both blastocysts and uterus. We wanted to know whether the endocannabinoid signalling system was present also in the sheep reproductive tract and which kind of effect(s) AEA had on the development of sheep blastocysts in vitro.AnimalsApoptosisApoptosis: drug effectsArachidonic AcidsArachidonic Acids: metabolismArachidonic Acids: pharmacologyBlastocystBlastocyst: drug effectsCB1CB1: metabolismCannabinoidCell ProliferationCell Proliferation: drug effectsEmbryonic DevelopmentEmbryonic Development: drug effectsEndocannabinoidsEndocannabinoids: metabolismFemaleIn Situ Nick-End LabelingNecrosisPolyunsaturated AlkamidesPolyunsaturated Alkamides: metabolismPolyunsaturated Alkamides: pharmacologyReceptorSheepSignal TransductionUterusUterus: metabolismHigh levels of anandamide, an endogenous cannabinoid, block the growth of sheep preimplantation embryos by inducing apoptosis and reversible arrest of cell proliferation.Article Journalhttp://humrep.oxfordjournals.org/cgi/content/abstract/23/10/233154dcce0a8580fe1368eeb329